qPCR Calculator for Edge

CRX id

phdljddfnhgkmldgfbmolkefobgmaada

Description from extension meta

An Edge extension for calculating qPCR ddCt values and ultimately gene expression levels.

Description from store qPCR Calculator is an Edge browser extension designed for molecular biology researchers, aimed at simplifying the analysis process of real-time quantitative PCR (qPCR) data. 1. **打开插件 / Open Plugin** - 点击浏览器工具栏中的插件图标，打开qPCR Calculator的主界面 Click the plugin icon in the browser toolbar to open the main interface of qPCR Calculator - 或者在任何页面右键，选择"qPCR Calculator" > "打开主界面" Or right-click on any page, select "qPCR Calculator" > "Open Main Interface" 2. **选择孔板类型 / Select Plate Type** - 在主界面中，选择您使用的孔板类型（96孔板或384孔板） In the main interface, select the plate type you are using (96-well or 384-well) 3. **添加样本属性 / Add Sample Properties** - **通过粘贴导入 / Import via Paste**：从Excel或CSV复制的数据格式，插件会自动识别并分配到对应的孔位 Copy data from Excel or CSV, the plugin will automatically recognize and assign it to the corresponding wells - **通过输入分配 / Assign via Input**：点击孔板上的特定孔位，弹出属性编辑窗口，填写样本名称、基因名称、CT值、重复编号等信息 Click on a specific well on the plate to open the property editing window, fill in sample name, gene name, CT value, replicate number, etc. 4. **确定复孔 / Determine Replicates** - 相同样本名称和基因名称的孔位会自动识别为复孔 Wells with the same sample name and gene name will be automatically recognized as replicates - 您可以在孔板上手动选择需要组合为复孔的孔位 You can manually select wells on the plate to be combined as replicates - 插件会自动计算复孔的平均CT值，提高数据可靠性 The plugin will automatically calculate the average CT value of replicates, improving data reliability 5. **设置对照和参考基因 / Set Control and Reference Gene** - 选择对照样本和参考基因，这些将用于ddCt值的计算 Select control samples and reference genes, which will be used for ddCt value calculation 6. **计算并查看结果 / Calculate and View Results** - 点击"计算"按钮，插件将自动计算ddCt值和基因表达水平 Click the "Calculate" button, the plugin will automatically calculate ddCt values and gene expression levels

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